Replacing Capillaries on a Standard Flow Cell

For bio-inert modules use bio-inert parts only!

When

If the capillary is blocked

Tools required

Qty.	p/n	Description
1		Wrench, 1/4 inch
1	5043-0915	Fitting mounting tool
1		Wrench, 4 mm
1		Screwdriver, Pozidriv #1 PT3

Parts required

Qty.		p/n	Description
1			For parts see Standard Flow Cell

Qty.

p/n

Description

For parts see

Standard Flow Cell

Prerequisites

Turn the lamp(s) off.
Open the doors of the module.
Remove the flow cell, see Remove and Install a Flow Cell.

NOTE

All descriptions in this procedure are based on the default orientation of the cell (as it is manufactured). The heat exchanger/capillary and the cell body can be fixed mirror symmetrically to have both capillaries routed to the bottom or to the top (depending on the routing of the capillaries to the column).

NOTE

The fittings at the flow cell body are special types for low dead volumes and not compatible with other fittings.

When retightening the fittings, make sure that they are carefully tightened (handtight plus 1/4 turn with a wrench). Otherwise damage of the flow cell body or blockage may result.

CAUTION

Sample degradation and contamination of the instrument

Metal parts in the flow path can interact with the bio-molecules in the sample leading to sample degradation and contamination.

For bio applications, always use dedicated bio parts, which can be identified by the bio-inert symbol or other markers described in this manual.
Do not mix bio, and non-bio modules or parts in a bio system.

Remove the outlet capillary.
To replace the inlet capillary, use a 4 mm wrench to open the fitting.
Unscrew the cell body from the heat exchanger.
Remove the heat exchanger from the clamp unit.
Use a small flat screw driver to carefully lift off the I.D. tag. Shown is the default orientation. See Note at the beginning of this section.
Unscrew the fixing screw and unwrap the inlet capillary from the grove in the flow cell body.
Take the new inlet capillary and bend it 90° about 35 mm from its end.
Bend the capillary again by 90° as shown below.
Insert the capillary into the hole between fixing screw and the inlet fitting.
The capillary lays in the grove and should be tied around the body (in the grove) 5 times.
Insert the fixing screw, so that the capillary cannot leave the grove.
Carefully insert the I.D. tag into the new heat exchanger. Shown is the default orientation. See Note at the beginning of this section.
Fix the heat exchanger to the clamp unit.
Fix the flow cell body to the heat exchanger.
Fix the inlet capillary to the flow cell body handtight first. Then do a 1/4 turn with a 4 mm wrench.
After replacing the outlet capillary, fix it handtight first. Then do a 1/4 turn with a 4 mm wrench.
Check for a centered holder vs. hole. If required adjust with the holder screws.
Reconnect the capillaries, see Remove and Install a Flow Cell.
Perform a leak test.
Insert the flow cell.
Close the doors of the module.
Perform a Wavelength Verification Test or a Holmium Oxide Test to check the correct positioning of the lamp.

21-Jun-2025