Wavelength Verification Test

The detector uses the alpha (656.1 nm) and beta (486 nm) emission lines of the UV-lamp for wavelength calibration. The sharp emission lines enable accurate calibration. When verification is started, the 1-nm slit is moved into the light path automatically. The test is run with Flow Cell removed or with Flow Cell installed.

If the test is performed with the Flow Cell removed, the test results are not influenced by solvent or pump effects.

When

The detector is calibrated at the factory, and under normal operating conditions should not require recalibration. However, it is advisable to recalibrate:
after repair of components in the optical unit,
after exchange of the optical unit or main board,
after replacing the Flow Cell or UV-lamp,
after significant environmental condition changes (temperature, humidity),
at a regular interval, at least once per year (for example, prior to an Operational Qualification/Performance Verification procedure), and
when chromatographic results indicate the detector may require recalibration.

Parts required

Qty.		p/n	Description
1			Removed Flow Cell

Prerequisites

Lamp must be on for at least 10 min.
When using a Flow Cell a flow rate of 0.5 mL/min with water is required.

Run the Wavelength Verification Test with the Agilent Lab Advisor (for further information see Online-Help of user interface).

Figure: Wavelength Verification – Results

21-Jun-2025