Holmium Oxide Test

The holmium oxide test uses characteristic absorbance maxima of the built-in holmium oxide filter to verify wavelength accuracy (see also Wavelength Verification Test). When the test is started, the 1-nm slit is moved into the light path automatically. To eliminate effects due to absorbing solvents, the test should be done with water in the flow cell or with removed flow cell.

Limits:

The test is evaluated by the instrument, and the measured maxima are displayed automatically. The test fails if one or more of the maxima lies outside of the limits (see Holmium Oxide Test ).