Replacing Capillaries on a Semi-Micro and Micro Flow Cell

When

If the capillary is blocked

Tools required

Qty.	p/n	Description
2		Wrench, 1/4 inch for capillary connections, or
1	5043-0915	Fitting mounting tool
1		Wrench, 4 mm for capillary connections
1		Screwdriver, Pozidriv #1 PT3

Parts required

Qty.		p/n	Description
1			For parts see or . Semi-Micro Flow Cell, or Micro Flow Cell

Qty.

p/n

Description

For parts see or .

Semi-Micro Flow Cell, or

Micro Flow Cell

Prerequisites

Turn the lamp(s) off.
Open the doors of the module.
Remove the flow cell, see Remove and Install a Flow Cell.

NOTE

All descriptions in this procedure are based on the default orientation of the cell (as it is manufactured). The heat exchanger/capillary and the cell body can be fixed mirror symmetrically to have both capillaries routed to the bottom or to the top (depending on the routing of the capillaries to the column).

NOTE

The fittings at the flow cell body are special types for low dead volumes and not compatible with other fittings. When retightening the fittings, make sure that they are carefully tightened (handtight plus 1/4 turn with a wrench). Otherwise damage of the flow cell body or blockage may result.

Identify the inlet and outlet capillaries.
Remove the outlet capillary.
To replace the inlet capillary, use a 4 mm wrench to open the fitting.
Unscrew the cell body from the heat exchanger.
Remove the heat exchanger from the clamp unit.
Use a small flat screw driver to carefully lift off the I.D. tag. Shown is the default orientation. See Note at the beginning of this section.
Carefully insert the I.D. tag into the new heat exchanger. Shown is the default orientation. See Note at the beginning of this section.
Fix the heat exchanger to the clamp unit.
Fix the flow cell body to the heat exchanger.
Fix the inlet capillary to the flow cell body handtight first. Then do a 1/4 turn with a 4 mm wrench.
After replacing the outlet capillary, fix it handtight first. Then do a 1/4 turn with a 4 mm wrench.
Check for a centered holder vs. hole. If required adjust with the holder screws.
Reconnect the capillaries, see Remove and Install a Flow Cell.
Perform a leak test.
Insert the flow cell.
Close the doors.
Perform a Wavelength Verification Test or a Holmium Oxide Test to check the correct positioning of the lamp.

21-Jun-2025