The most important parameters of the pump are listed in the following table.
Parameter | Limits | Description |
|---|---|---|
| 0.001 – 5 mL/min | Total flow rate of the pump. See When to Remove Damper and Mixer for pump hardware modifications to achieve lowest delay volume. |
| 0.01 min - no limit | The stop time of the pump usually controls the run time of the whole LC system. Use no limit to stop the run manually (useful for method development). |
| off - 99999 min | Time between the end of a run and the start of the next. Used for column equilibration after a gradient. |
| Max: 0 – 600 bar Min: 0 – 600 bar | Max must be bigger than Min! Set max pressure to the maximum operating pressure of your column. A min pressure setting of e.g. 10 bar will turn off your pump automatically when running out of solvent. A smarter way, however, is to use the bottle fillings function (see Bottle Filling). |
| 0 – 100 % | Although channel A can be set to 0 %, it cannot be turned off. This channel should be used for the aqueous phase (water). |
| off - 100 % | The percentage of channel B is automatically complemented by channel A to give 100 %. |
| H₂O, ACN, MeOH, IPA | Select the solvent you are using in the respective solvent channel from the drop-down list. In case your solvent is not listed, perform a solvent compressibility calibration. |
|
| Free text field for a description of the solvent. This description will show up in method printouts, etc. |
| max. number of lines depends on free space in pump memory | Use the timetable to build solvent gradients, flow gradients, or combinations of both. Gradients are always linear. Use multiple timetable entries to mimic exponential or parabolic gradients. |
|
| There are three ways to display the timetable:
Values can only be changed in tabular view. |
| 0.001 – 5 mL/min | Total flow rate of the pump. |
| 0.01 min - no limit | The stop time of the pump usually controls the run time of the whole LC system. Use no limit to stop the run manually (useful for method development). |
| off - 99999 min | Time between the end of a run and the start of the next. Used for column equilibration after a gradient. |
| Max: 0 – 600 bar Min: 0 – 600 bar | Max must be bigger than Min! Set max pressure to the maximum operating pressure of your column. A min pressure setting of e.g. 10 bar will turn off your pump automatically when running out of solvent. A smarter way, however, is to use the bottle fillings function (see Bottle Filling). |
| 0 – 100 % | Although channel A can be set to 0 %, it cannot be turned off. This channel should be used for the aqueous phase (water). |
| off - 100 % | The percentage of channel B is automatically complemented by channel A to give 100 %. |
| H₂O, ACN, MeOH, IPA CO²pre-compressed | Select the solvent you are using in the respective solvent channel from the drop-down list. In case your solvent is not listed, perform a solvent compressibility calibration. |
|
| Free text field for a description of the solvent. This description will show up in method printouts, etc. |
| max. number of lines depends on free space in pump memory | Use the timetable to build solvent gradients, flow gradients, or combinations of both. Gradients are always linear. Use multiple timetable entries to mimic exponential or parabolic gradients. |
|
| There are three ways to display the timetable:
Values can only be changed in tabular view. |
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