The refractive index calibration is based on a sucrose calibration solution, which has a known refractive index compared to LC grade water. After both the sample and reference cells have been purged with LC grade water the sucrose solution is introduced into the flow cell and then the built-in refractive index calibration functionality is used.
Filling the sample cell with the sucrose calibration solution will give a theoretical detector response of 512,000 nRIU +/- 5,000 nRIU. The calibration algorithm will allow the actual detector response, if different, to be changed to the theoretical value.
NOTE
Refractive index calibration is only required after exchange of the optical unit or the main (RIM) - board.
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