Preparing the Calibration

Preparation of the sucrose calibration solution (final concentration: 3.5 g/L).

1. To prepare 25 ml of the calibration solution 87.5 mg of the Sucrose sample is required.

2. Add the weighed amount of sample into a suitable volumetric flask.

3. Dispense 10 ml of LC grade water into the flask and shake or stir to dissolve.

4. Dilute the contents of the flask to volume with LC grade water.

Wait five minutes and shake again. The solution is now ready for use.

Preparing the pump.

1. Fill a suitable solvent bottle with LC grade water.

2. Connect this bottle to Channel A of the pump, A1 if a binary pump.

Flush the degasser and pump.

Run the RID Tools Screen with the Agilent Lab Advisor (for further information see Online-Help of user interface). (If an Agilent pump is part of the system, the pump section is active.)

Purging the sample and reference cells.

1. The purge valve will automatically switch to the ON position.

2. Using a syringe or LC pump flush the sample and reference cell with about 20 mL of LC grade water. (If an Agilent pump is part of the system, the pump section is active).

3. The purge valve will automatically switch to the OFF position when you click continue.

Fill the sample cell with calibration solution.

1. Take the syringe and fix the needle to the syringe adapter.

2. Suck about 2 mL of the calibration sample into the syringe.

3. Keep the syringe in a horizontal position.

4. Remove the needle.

5. Add the filter to the syringe and eject the calibration sample to waste (through the filter). This will ensure removing any particles from the filter.

6. Remove the filter.

7. Take the syringe and fix the needle to the syringe.

8. Suck about 4 mL of the calibration sample into the syringe.

9. Keep the syringe in a horizontal position.

10. Remove the needle.

11. Add the filter to the syringe and fit the needle to the filter.

12. Lift the needle tip and carefully eject approximately 0.5 mL to remove air out of the syringe.

Leave the RID Tools Screen.