Setting Up the Dual-Needle System With OpenLab ChemStation
A right-click into the Active Area will open a menu to
Show the Control User Interface (special module settings)
Show the Method User interface (same as via menu Instrument > Set up Instrument Method > Setup G7167B )
Injector Program
When you activate a pretreatment/injector program, it replaces the standard injection cycle.
Identify Device
Home All
Reset Injector
Switch to Mainpass (main path) Left (needle loop left is connected to the pump device)
Switch to Mainpass (main path) Right (needle loop right is connected to the pump device)
Switch on Tray Illumination
Auto-clean
Prime Solvents
Start Purge:
Manual start of the purge routine, duration defined by hydraulic setup.
See Purge for further information on purge.
Modify
Drawer Configuration: Changing the load capacity of the Sample Hotel
Capillaries Setup: for the sample loops, needle seat, and bypass capillaries for dual-needle option
Reference Vial Bar
Assign Wellplates
Wellplate Configuration (same as click on the Tray icon)
Purge
Typical time for purge:
1290 Binary pump, 2 x 20 µL setup, flow rate: 0.5 mL/min ~ 125 s
1290 Binary pump, 2 x 20 µL setup, flow rate: 1.0 mL/min ~ 85 s
Other configurations (especially large volume setups) will last longer.
In order to get information about remaining purge time, expand the window in the user interface:
NOTE
The start of the pump or changes in solvent composition trigger the purge routine of the multisampler. The purge routine flushes the hydraulic setup of the multisampler with fresh mobile phase (for example metering device, sample loops, and needles). This ensures cleanness of the flowpath.
NOTE
For pumps with a manual purge valve, it is mandatory to start the purge routine before a run or sequence. This will guarantee that the complete flow path of the dual-needle setup is flushed with fresh mobile phase.
NOTE
The only way to speed up the purge routine is to increase flow rate. Best practices is to write a purge method in the sequence table. The purge method includes a column switching valve that switches at high flow rate into the waste position.
Modify Capillaries
NOTE
To avoid damage of the system, the configuration of the dual-needle system must match to the installed hardware, especially the sample loops.
NOTE
Only the listed capillary PN 5500-1238 can be used as bypass capillary (either left or right).
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