Method Setup

Alternating Needle: Needles will be toggled (only possible if the both flowpaths of are configured identically)

Right Needle: only the right needle will be used

Left Needle: only the left needle will be used

Draw Speed: determines the rate at which the plunger draws sample from the vial. Set the speed to an appropriate value for your sample. For viscous samples, use a slow Draw Speed.

Eject Speed: determines the rate at which the plunger ejects sample from the metering device. If you are injecting large volumes of sample, setting a high Eject Speed will shorten the time needed for an injection cycle. For viscous samples, use a slow Eject Speed.

Wait Time After Draw: this time ensures that the temporary vacuum, which originates from the drawing of liquid from the sample vial, dissipates. The needle first stays on the seat for the specified time, then after drawing sample from the vial remains there for the specified time.

Offset: this is a vertical offset that enables you to position the needle a specific distance (in mm) away from its standard position. The Offset function is useful when analyzing very small sample volumes, or when only a specific part of the sample is required, for example, the top layer.

Usually default draw offset = 0 equates to 2 mm above the wellplate bottom.

Use Vial/Well Bottom Sensing: this feature allows the needle to detect non-uniform well bottoms, and adjusts the depth of the needle position to 2 mm (default value) above the detected bottom of the vial or well. You can use Vial/Well Bottom Sensing in combination with the Offset to customize the draw position of the needle.To turn on Vial/Well Bottom Sensing, mark the check box. The default setting is cleared. You may want to turn off Vial/Well Bottom Sensing to increase speed of injection, or to avoid the needle touching the bottom of the well if a sample precipitate could clog it.

Enable Smart Overlap: Overlapped injection provides faster throughput of samples by allowing the preparation of the next injection while the current injection is in the mainpass (main path).

This section is available only for a dual-needle Multisampler with identical left and right flow paths (Seat capillary and Loop capillary), and Alternating Needle selected in the Injection section of the method setup.

After Period of Time: specifies the time (in minutes) that the Multisampler waits after injection of a sample before taking up and injecting the next sample.

NOTE: It is important to calculate the time close to the start point of the next run to avoid waiting time with filled sample loop.