Replacing Capillaries on a Standard Flow Cell
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生物惰性模块仅使用生物惰性部件！ |
适用于
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所需工具
数量 | 部件号 | 描述 | |
|---|---|---|---|
1 | 
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| 扳手，1/4 英寸 |
1 | | 5043-0915 | 接头安装工具 |
1 |
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| Wrench, 4 mm |
1 |
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| 米字螺丝刀 #1 PT3 |
所需部件
数量 | 部件号 | 描述 | |
|---|---|---|---|
1 |
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| For parts see |
前提条件
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注意
All descriptions in this procedure are based on the default orientation of the cell (as it is manufactured). The heat exchanger/capillary and the cell body can be fixed mirror symmetrically to have both capillaries routed to the bottom or to the top (depending on the routing of the capillaries to the column).
注意
The fittings at the flow cell body are special types for low dead volumes and not compatible with other fittings.
When retightening the fittings, make sure that they are carefully tightened (handtight plus 1/4 turn with a wrench). Otherwise damage of the flow cell body or blockage may result.
小心
样品降解和仪器污染
流路中的金属部件可能与样品中的生物分子发生反应，这会导致样品降解和污染。
- 对于生物应用，请始终使用生物部件，使用本手册所述的生物惰性标志或其他标志可以识别出这种部件。
- 请勿在生物系统中混用生物和非生物模块或部件。
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Remove the outlet capillary.
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To replace the inlet capillary, use a 4 mm wrench to open the fitting.
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Unscrew the cell body from the heat exchanger.
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Remove the heat exchanger from the clamp unit.
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Use a small flat screw driver to carefully lift off the I.D. tag. Shown is the default orientation. See Note at the beginning of this section.
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Unscrew the fixing screw and unwrap the inlet capillary from the grove in the flow cell body.
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Take the new inlet capillary and bend it 90° about 35 mm from its end.
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Bend the capillary again by 90° as shown below.
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Insert the capillary into the hole between fixing screw and the inlet fitting.
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The capillary lays in the grove and should be tied around the body (in the grove) 5 times.
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Insert the fixing screw, so that the capillary cannot leave the grove.
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Carefully insert the I.D. tag into the new heat exchanger. Shown is the default orientation. See Note at the beginning of this section.
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Fix the heat exchanger to the clamp unit.
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Fix the flow cell body to the heat exchanger.
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Fix the inlet capillary to the flow cell body handtight first. Then do a 1/4 turn with a 4 mm wrench.
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After replacing the outlet capillary, fix it handtight first. Then do a 1/4 turn with a 4 mm wrench.
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Check for a centered holder vs. hole. If required adjust with the holder screws.
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Reconnect the capillaries, see 取出和安装流通池.
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Perform a leak test.
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Insert the flow cell.
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Close the doors of the module.
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id: 3358984715