Depending on the ambient conditions and the sampler configuration (for example, hotel configuration for the Multisampler), reaching the setpoint temperature can take from 30 min up to a couple of hours.
NOTE
Reaching the 4 °C setpoint from an ambient temperature of 22 °C takes about 45 min for the Vialsampler (G7129A/B/C or G7157A), as well as for the Multisampler (G7167A/B, G5668A, G7137A, or G4767A), and the Online Sample Manager (G3167A) with a single 2H drawer installed.
NOTE
This relatively slow ramping down of the temperature is necessary to avoid ice formation.
NOTE
For the best performance of the Sample Cooler/Sample Thermostat, all drawers must be installed in the sampler. For the Multisampler, use dummy drawers if no full hotel configuration is needed.
Operating the thermostat at temperatures below ambient results in condensate formation. This condensed water is collected in the base plate of the thermostat and drained through the drainpipe at the back of the unit. The container for condensate collection should be regularly emptied to ensure the proper functioning of the system.
NOTE
If the container is overfilled or the condensate tubing is blocked, the condensate sensor is triggered, rendering the HPLC system to enter the error state (see Sample Temperature Control Switched Off Due to Condensate).
NOTE
Depending on the ambient conditions in the lab, the amount of condensate can vary from 200 mL to 2 L per day. Waste containers for the condensate should not be filled to the top. The waste container must be emptied regularly.
Setting the thermostat from a lower to a higher temperature setpoint, or just simply turning it off, can result in dew formation on the internal surfaces of the sampler. This is normal and should cease after a couple of hours at the most.
Opening the door(s) and/or the sample drawers frequently can compromise the temperature stability, as fresh warm and humid air will enter each time. In a highly humid environment, this could also lead to the formation of significant amounts of condensate on the internal surfaces of the sampler.
The Sample Thermostat was designed to operate without the risk of icing. In an unlikely event of ice formation, turn off the thermostat and wait until it defrosts.
NOTE
Do not use mechanical devices or other means to accelerate the defrosting process.
When the Sample Thermostat needs to be turned off for the night or a longer period, the following best practices are recommended:
Remove all sample containers and/or vials from the sampler.
Let the system reach the ambient temperature. Opening the door(s) of the sampler facilitates this process.
Remove any condensate that might appear on the sample drawers or the internal surfaces of the sampler.
Make sure that all condensate is removed from the thermostat.
NOTE
Gently tapping on the sides of the sampler facilitates the condensate removal. Tilting the module towards its right back corner is not recommended as it can damage the internal parts.
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