Method Parameter Settings

The Quaternary Pump method setup parameters are in nine sections:

Flow
Solvents A to D
Stoptime
Posttime
Pressure Limits
Timetable
Advanced
Blend Assist
ISET

**Table:** Method parameters
Parameter	Limits	Description
Flow	0.00 – 5.00 mL/min in steps of 0.001 . Recommended flow range: 0.05 – 5.00 mL/min.	The flow is the rate of movement of eluent along the column. It is important that the flow rate is kept constant to ensure precise retention time, and peak measurements. Variations in flow rate can occur as a result of the partial failure of the pumping system, air in the pumping system, a change in the mobile phase viscosity or a temperature change.
Enable Blend Assist		Mark this check box to switch on Blend Assist, which allows you to set up solvent mixtures from stock solutions. When this check box is marked, the Blend Assist section of the method setup is available.
Solvents		Blend Assist Disabled: When Blend Assist is disabled, you can set the percentages of solvents B, C, and D to any value from 0 through 100 %. Solvent A always delivers the remaining volume: 100 - (%B + %C + %D). The check boxes allow you to turn on the solvent channels (checked) or off (cleared). Click the solvent name down arrow and select the solvent from the list of calibrated solvents and solvent mixtures. For solvent mixtures, specify the percentage of additive. You can enter your own name for the solvent or solvent mixture in the adjacent field. Blend Assist Enabled: When Blend Assist is enabled, the table shows the solvent blends that have been set up in the Blend Assist section of the method setup. Solvent: The solvent or blend of solvents as set up in the Blend Assist section. Used: Mark this check box if you want to use this solvent or blend in the method. %: Enter the percentage of the solvent or blend in this field. Name: Type a name for the solvent or blend in this field.
Stoptime	0.01 – 99999 min or As Injector/No Limit (an infinite run time).	The stoptime sets a time limit for your analysis. After the stoptime, all gradients are stopped and the pump parameters return to their initial values. The pump can be used as a stoptime master for the complete analytical system. If they have a No Limit stoptime setting, the pump also stops the detectors. If no limit is given, a method will have to be stopped manually.
Posttime	0.01 – 99999 min or Off (0.0 min).	Your instrument remains in a not ready state during the posttime to delay the start of the next analysis. You can use the Posttime to allow your column to equilibrate after changes in solvent composition (for example after gradient elution).
Pressure Limits	Max: 1300 bar (18850 psi) for flow rates up to 2 mL/min. For flow rates between 2 mL/min and 5 mL/min, the maximum pressure ramps down to 800 bar (11600 psi). Min: any value between 0 and the upper pressure limit setting.	Sets the maximum and minimum pressure limits for the pump. Max is the maximum pressure limit at which the pump will switch itself off, protecting the analytical system against overpressure. Min is the minimum limit at which the pump will switch itself off, for example, if any solvent reservoir is empty, this prevents system damage by pumping air.
Timetable		See Timetable Settings
Advanced		See Advanced Settings
ISET		Set the ISET parameters in this dialog box. For further information on ISET, please see the Online Help or to the Agilent InfinityLab LC with ISET User Manual (G7120-ISET-SysMa-en-SD-29002093.pdf, SD-29002093).
Flow	0.00 – 5.00 mL/min in steps of 0.001 . Recommended flow range: 0.05 – 5.00 mL/min.	The flow is the rate of movement of eluent along the column. It is important that the flow rate is kept constant to ensure precise retention time, and peak measurements. Variations in flow rate can occur as a result of the partial failure of the pumping system, air in the pumping system, a change in the mobile phase viscosity or a temperature change.
Enable Blend Assist		Mark this check box to switch on Blend Assist, which allows you to set up solvent mixtures from stock solutions. When this check box is marked, the Blend Assist section of the method setup is available.
Solvents		Blend Assist Disabled: When Blend Assist is disabled, you can set the percentages of solvents B, C and D to any value from 0 through 100 %. Solvent A always delivers the remaining volume: 100 - (%B + %C + %D). The check boxes allows you to turn the solvent channels on (checked) or off (cleared). Click the solvent name down arrow and select the solvent from the list of calibrated solvents and solvent mixtures. For solvent mixtures, specify the percentage of additive. You can enter your own name for the solvent or solvent mixture in the adjacent field. Blend Assist Enabled: When Blend Assist is enabled, the table shows the solvent blends that have been set up in the Blend Assist section of the method setup. Solvent: The solvent or blend of solvents as set up in the Blend Assist section. Used: Mark this check box if you want to use this solvent or blend in the method. %: Enter the percentage of the solvent or blend in this field. Name: Type a name for the solvent or blend in this field.
Stoptime	0.01 – 99999 min or As Injector/No Limit (an infinite run time).	The stoptime sets a time limit for your analysis. After the stoptime, all gradients are stopped and the pump parameters return to their initial values. The pump can be used as a stoptime master for the complete analytical system. The pump also stops the detectors if they have a No Limit stoptime setting. If no limit is given, a method will have to be stopped manually.
Posttime	0.01 – 99999 min or Off (0.0 min).	Your instrument remains in a not ready state during the posttime to delay the start of the next analysis. You can use the Posttime to allow your column to equilibrate after changes in solvent composition (for example after gradient elution).
Pressure Limits	Max: 800 bar (11600 psi) for flow rates up to 5 mL/min. Min: any value between 0 and the upper pressure limit setting.	Sets the maximum and minimum pressure limits for the pump. Max is the maximum pressure limit at which the pump will switch itself off, protecting the analytical system against over-pressure. Min is the minimum limit at which the pump will switch itself off, for example, if any solvent reservoir is empty, this prevents system damage by pumping air.
Timetable		See Timetable Settings
Advanced		See Advanced Settings
ISET		Set the ISET parameters in this dialog box. For further information on ISET, please refer to the Online Help or to the Agilent InfinityLab LC with ISET User Manual (G7120-ISET-SysMa-en-SD-29002093.pdf, SD-29002093).

Advanced Settings

The Quaternary Pump advanced method setup parameters are in five sections:

Minimum Stroke
Compressibility
Maximum Flow Gradient
Primary Channel
Mixer Selection

**Table:** Advanced method parameters
Parameter	Limits	Description
Minimum Stroke	20 – 100 µL	The Stroke Volume is used for optimizing between performance of the module and seal life time. For performance, a low stroke volume is beneficial, as it divides disturbances into smaller packages, but a larger volume is extending the life time of the pump seals. If Automatic is activated, the pump tries to achieve an optimized stroke volume for the Inline Weaver geometry.
Compressibility		The compressibility of the mobile phase affects the performance of the pump. For best flow accuracy and mixing performance, you can set the parameter according to the mobile phase being used. Use solvent types: Select this check box (recommended) for using the enhanced and automatic compressibility calibration. Then select the calibrated solvent from the drop-down lists using the combo boxes in the Solvents section. Using this check box hides compressibility fields for manual settings. Clear this check box to display the compressibility fields, which allow you to enter manual compressibility values, which are constant over pressure. This setting is available for method backward compatibility, e.g. from 1260 Infinity pumps. For best performance, use solvent types.
Maximum Flow Gradient	1.000 - 1000.000 mL/min/min in steps of 0.001 mL/min/min Default value: 100.000 mL/min/min	You can set a limit on the rate of change of the solvent flow to protect your analytical column. You can set individual values for Flow ramp up and Flow ramp down.
Primary Channel		Using Automatic is recommended. The primary channel can be specified as A to D for optimizing highly specific methods. It is split up to deliver the first and last solvent package created by the MCGV in order to optimize composition precision. The primary channel does not change during a gradient, as long as the channel is used. Using Automatic chooses the channel with the highest percentage at start conditions before a gradient.
Mixer Selection		Click the down-arrow and select the mixer to use from the list: Use any mixer: The currently installed mixer is used, irrespective of its type. Do not use mixer: The valve is set to bypass the mixer so that it is not in the flow path. <Mixer Name>: Only the specified mixer may be used; it the mixer is not found, the pump goes into a Not Ready state.

Timetable Settings

Use the Timetable to program changes in the pump parameters during the analysis by entering a time in the Time field and appropriate values in the following fields of the timetable. Changes in flow rate occur linearly from either time zero or the time of the last defined change; other parameters change instantaneously at the time defined in the timetable.

Show that Advanced Timetable toggles the timetable display between standard mode and advanced mode.

The following parameters can be changed:

Change Contacts
Change Flow
Change Max. Pressure Limit
Change Solvent Composition - You can only use solvents, which have been enabled in the solvents section.
Function centric view - This check box allows you to display parameter changes instead of a time table.

Blend Assist

The Blend Assist table allows you to blend two or more solvents or solvent mixtures from stock solutions. The blends must be of pure solvents or pure solvents with additives. For example, you can blend 100 % water with 10 % isopropanol in water.

Channel: The channel name.
Type: The type of solvent
- Solvent <n>: Pure solvent
- Solvent <n> Additive: Solvent mixture
Calibration: Click the down arrow and select the solvent or solvent mixture from the list.
Name: Enter a name for the solvent or solvent mixture in this field.
Stock conc.: For solvent mixtures, specify the concentration of the additive in the stock solution in this field. Pure solvents are always 100 %.
Final conc.: Enter the concentration of the additive that you want to achieve in this field. The pure solvent and solvent mixture will be blended to achieve the Final conc. For the relationship of stock concentration and concentration in the mixture, the composition accuracy needs to be considered (see Performance specifications of the 1290 Infinity III Flexible Pump (G7104A) or Performance specifications of the 1290 Infinity III Bio Flexible Pump (G7131A)).
Final conc.: Enter the concentration of the additive that you want to achieve in this field. The pure solvent and solvent mixture will be blended to achieve the Final conc. For the relationship of stock concentration and concentration in the mixture, the composition accuracy needs to be considered (see Performance specifications of the 1260 Infinity III Flexible Pump (G7104C) or Performance specifications of the 1260 Infinity III Bio Flexible Pump (G7131C)).
Conc. unit: The concentration can be defined as mM (mmol/L) or as %.

21-Jun-2025