Repairing the Flow Cells

Parts required

Qty.		p/n	Description
1			Flow cell

For details on flow cells see:

NOTE

The shown cell parts will differ depending upon the flow cell type. For detailed parts schematics, refer to above mentioned pages.

1	Cell screw	2	Conical springs
3	Ring #1 PEEK	4	Window Quartz
5	Gasket #1 (small hole)	6	RFID tag
7	Gasket #2 (large hole)	8	Ring #2 PEEK

Disassembling the Flow Cell.
1. Unscrew the cell screw using a 4-mm hexagonal wrench.
1. Remove the SST rings using a pair of tweezers.
CAUTION
Scratched window surfaces by tweezers
Window surfaces can easily be scratched by using tweezers for removing the windows.
- Do not use tweezers to remove windows
1. Use adhesive tape to remove the peek ring, the window and the gasket.
1. Repeat step a through step c for the other window (keep the parts separate - otherwise they could be mixed!).
Cleaning the Flow Cell Parts
1. Pour isopropanol into the cell hole and wipe clean with a piece of lint-free cloth.
1. Clean the windows with ethanol or methanol. Dry it with a piece of lint-free cloth.
NOTE
Always use new gaskets.
Reassembling the Flow Cell
1. Hold the flow cell cassette horizontally and place gasket in position. Ensure both cell holes can be seen through the holes of gasket.
NOTE
The semi-micro #1 and #2 gaskets (items 6 and 7, Semi-micro Flow Cell (Parts)) look very similar. Do not mix them up.
1. Place the window on gasket.
1. Place the peek ring on the window.
1. Insert the conical springs. Make sure the conical springs point towards the window. Otherwise tightening the cell screw might break the window.
Figure: Orientation of conical springs
1. Screw the cell screw into the flow cell and tighten the screw.
Repeat the procedure for the other cell side.
Reconnect the capillaries.
Perform a leak test. If OK, insert the flow cell.
{Comment by , : ToDo: Condition: "not(($ProductGroup='G7114B'))" }Perform --- MISSING LINK --- to check the correct positioning of the flow cell.
Perform Wavelength Calibration to check the correct positioning of the flow cell.
Replace the front cover.

21-Jun-2025