Probable cause | Suggested actions | |
---|---|---|
1 | Ensure that the method settings are properly set and the gradient system is delivering the correct gradient composition. Check if the mobile phase reservoirs are empty or if the filter frits are blocked. Check for air bubbles in the system. If necessary, purge the pump and system with a viscous mobile phase to remove any air bubbles. Check the system for any leaks. Check for leaking pump seals. If necessary, replace pump seals. Check for sticking check valves. If necessary, sonicate or replace check valves. | |
2 | Changing mobile phase composition | Ensure proper mobile phase preparation. Ensure the gradient system is delivering the correct gradient composition. For isocratic applications, premix the mobile phase. |
3 | Replace the column if necessary. | |
4 | Improper method settings | Check temperature settings in the column compartment. |
base-id: 4303170699
id: 4303170699