Negative peaks

Subpages

Probable cause	Suggested actions
1	Mobile phase absorption/refraction too high (UV Detectors)	Use less absorptive/refractive solvents or mobile phase additives. Change UV wavelength or switch the detector polarity .
2	Injection solvent effects	If possible, use the starting mobile phase composition or weaker solvents as the injection solvent. Ensure that the injection solvent is miscible with the mobile phase and causes no salt precipitation.
3	Improper method settings	Check the detector method settings , such as wavelength and data rate, and optimize them if needed.

Probable cause

Suggested actions

Mobile phase absorption/refraction too high (UV Detectors)

Use less absorptive/refractive solvents or mobile phase additives.

Change UV wavelength or switch the detector polarity .

Injection solvent effects

If possible, use the starting mobile phase composition or weaker solvents as the injection solvent.

Ensure that the injection solvent is miscible with the mobile phase and causes no salt precipitation.

Improper method settings

Check the detector method settings , such as wavelength and data rate, and optimize them if needed.