Method Parameter Settings

The method parameter settings are available via right click on the active area of the grafical user interface.

**Table:** Method parameter settings
	Injection Specify the injection volume (in µL) in the Injection Volume field. The injection volume limits depend upon the configuration settings of the autosampler. Note that this setting is ignored when Pretreatment/Injector Program is defined.
	Needle Wash Mark the Enable Needle Wash check box to specify an injection with needle wash. The following wash parameters can be specified: Mode: Select the wash location by clicking the down arrow and selecting the needle wash mode from the list. You can select from Flush Port (default location) or Wash Vial. Time: This field is enabled only if you have selected Flush Port. Specify a flush time in seconds between 1 and 100 in steps of 1. Location: enabled only if you have selected a Wash Vial. Allows you to specify a wash location in the format Vial X, where X is the vial number. Specify well locations in the format P<Plate #>-<Row Character><Column Number>, for example, P1-A1. The values of <Row Character> and <Column Number> depend on the wellplate type. Repeat: available only if you have selected Wash Vial. You can specify up to 10 repeat washes.
	Stoptime: enables you to set the time that the analysis stops. Limits: 0.01 through 99999 min or As Pump/No Limit. Posttime: the Vialsampler remains in a post-run state during the Posttime to delay the start of the next analysis. A Posttime period can be used to allow the column to equilibrate after changes in solvent composition (for example after gradient elution). Limits: 0.01 through 99999 min or Off.
	Auxiliary Draw Speed: determines the rate at which the plunger draws sample from the vial. Set the speed to an appropriate value for your sample. For viscous samples, use a slow Draw Speed. Eject Speed: determines the rate at which the plunger ejects sample from the metering device. If you are injecting large volumes of sample, setting a high Eject Speed will shorten the time needed for an injection cycle. For viscous samples in combination with multidraw, use a slow Eject Speed. Wait Time After Draw: ensures that the temporary vacuum, which originates from the drawing of liquid from the sample vial, dissipates. The needle stays first on the seat for the specified time, then after drawing sample from the vial, remains there for the specified time. Needle Height Offset: a vertical offset that enables you to position the needle a specific distance (in mm) away from its standard position. The Offset function is useful when analyzing very small sample volumes, or when only a specific part of the sample is required, for example, the top layer.
	High Throughput Injection Valve to Bypass for Delay Volume Reduction: Mark this check box to switch the flow from the injector from mainpass (main path) to bypass after injection has taken place. This reduces the delay volume for low volume techniques. You can specify the point during the analyses when the valve switches to bypass by setting the Sample Flush-out Factor. Sample Flush-Out Factor: ensures that the sample is thoroughly flushed out of the syringe and past the capillary seat and valve. The Sample Flush-Out Factor is preset to 5.0 at the factory. The preset Sample Flush-Out Factor is correct for most methods. However, for unusually viscous samples, you should increase the Sample Flush-Out Factor to obtain the desired degree of flushing to prevent sample carryover. Overlapped Injection Mode: provides faster throughput of samples by allowing the preparation of the next injection while the current injection is being completed. The following options can be selected from the drop-down list: Off: to switch off overlapped injection. Select When sample is Flushed Out to specify that the sample can be prepared directly after the current sample has been flushed out. Prefetch Vial: to specify that the next sample is fetched by the gripper while the current sample is running. Overlap Injection Cycle: to specify that the next sample is fetched and loaded into the sample loop while the current sample is running. Specify a time (in minutes) after injection when this process should be started.

NOTE

For additional help and support, highlight the desired area in the user interface and press the F1 key. A help screen will open with additional information and documentation about the topic.

21-Jun-2025