The Optical Balance Procedure

When

When light falling on light receiving diodes is out of balance.

Tools required

Qty.		p/n	Description
1			Flat head screwdriver

NOTE

This procedure should only be performed to correct a permanent misalignment of the light beam that cannot be eliminated by flushing the sample and the reference cell with the same solvent and by equilibrating the system.

Run the RID Tools Screen with the Agilent Lab Advisor (for further information see Online-Help of user interface). (If an Agilent pump is part of the system, the pump section is active.)
Purging the sample and reference cells.
1. Switch the purge valve to the ON position.
1. Purge the sample and reference cells for around 10 min with the solvents to be used.
1. Switch the purge valve to the OFF position
Select Optical Balance.
Adjust Optical Balance.
1. While monitoring the optical balance use the flat-headed screwdriver to turn the zero glass adjustment screw slowly.
1. When the diode balance value reaches 0.00 (green area), optical balance is restored.
Figure: Turning the zero glass adjustment screw

21-Jun-2025