When the sample and reference cells both contain the same liquids an equal amount of light should fall on each light receiving diode, the diode balance will equal 0. If this balance of light needs to be corrected the optical balance procedure can be used.
Diode balance is calculated as follows:
Where:
diode1 = signal proportional to the amount of light falling on diode1
diode₂ = signal proportional to the amount of light falling on diode₂
Optical balance adjustment is a manual procedure where the position of the light beam falling on the light receiving diode is adjusted using the zero glass adjustment screw.
NOTE
The detector will become not-ready when the diode balance value falls outside the range - 0.5 to + 0.5.
NOTE
Both sample and reference cell must be purged with the same solvent before optical balance is performed. Prior to performing this procedure, the system must be well equilibrated.
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id: 3596138251