Using the Agilent Lab Advisor
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Set up the HPLC system and the Agilent Lab Advisor.
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Flush the flow cell with clean bi-distilled water.
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Turn on the FLD lamp.
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Run the Wavelength Accuracy Test.
The FLD will change into the multi-excitation mode with emission wavelength at 397 nm and scan in the range of the expected maximum of 350 nm ± 20 nm.
As result, the maxima should be found at 350 nm ± 3 nm, see following figure.
The FLD will change into the multi-emission mode with excitation wavelength at 350 nm and scan in the range of the expected maximum of 397 nm ± 20 nm.
As result, the maxima should be found at 397 nm ± 3 nm, see following figure.
Figure: Excitation and Emission Spectrum (expected results) NOTE
If the plots do not have a maximum around EM=397 nm and EX=350 nm (± 3 nm) the test fails. See Interpretation of the Results.
If the test fails observe the maxima of the EX or EM side under the Signals tab.
If the plots do not have a maximum around EX=397 nm and EX=350 nm (±3 nm) the test fails, see figure below. Refer to Interpretation of the Results.
base-id: 3586775947
id: 3586775947