If the test fails, check for:
correctly positioned flow cell,
clean flow cell (flush with isopropanol and clean bi-distilled water),
no air bubble(s) (check via fluorescence scan or visual check of cell/cuvette),
solvent inlet filter (may create air bubbles in flow cell).
check optical path for contamination (service)
check alignment of lamp / trigger pack assembly (service)
perform a Wavelength Calibration
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id: 3586771467