The geometrical volume of the 60 mm cell is 6 times larger than the 10 mm cell. However, the chromatographic relevant dispersion volume, the square roots of variances, accounting for cell specific geometrical volume shape and fluidic flow pattern, have been determined as σV = 4 µL and σV = 1 µL in for the 10 mm cell.
Due to the larger dispersion volume, the 60 mm cell is primarily designed for 4.6 mm column applications to achieve highest sensitivity with no additional peak broadening. However, if sensitivity is important the 60 mm cell will also be advantageous in case of smaller columns (3 mm, 2.1 mm) but depending on the chromatographic system and method additional peak broadening might occur.
Care should be taken in methods where high background absorption of solvents or modifiers are present. When using the 60 mm cell the detector will measure 6 times the background absorption as in case of the 10 mm cell, which will reduce the remaining dynamic absorbance range for sample peaks. Furthermore those UV absorbing modifiers could compromise the sensitivity gain (signal/noise) of 60 mm cell.
The linearity limit of the detector is seen at about 2 AU for both, the 10 mm and the 60 mm Max-Light Cartridge Flow Cell. Using firmware revision B.06.25 and below, the 60 mm Max-Light Cartridge Cell linearity limit would be 333 mAU/cm.
base-id: 3526189323
id: 3526189323