1 Selection of flow cell | |
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2 Connection of flow cell | |
3 Setting the peak width (response time) | |
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4 Setting wavelength and bandwidth | |
Sample wavelength: Never miss a peak by the use of a browser wavelength like 250 nm with 100 nm bandwidth. Select specific wavelength with reduced optical bandwidth if you need selectivity, e.g. 254.0 nm / 4 nm and 360.0 nm / 100 nm as reference wavelength. Set the sample wavelength to a peak or valley to get best linearity in general; select a valley to get best linearity for high concentrations.
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Reference wavelength: Select the reference wavelength with broad bandwidth (30...100 nm) wavelength range where your analytes have little or no absorbance (e.g. sample at 254 nm, reference at 320 nm). Select the reference wavelength as near as possible to the UV range.
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5 Setting the slit width (G7117B only) | |
Use 4 nm slit for normal applications. Use narrow slit (e.g 1 nm) if your analytes have narrow absorbance bands and for high concentrations. Use a wide slit (e.g. 8 nm) to detect very low concentrations. Optimizing spectral acquisition (DAD only) Set the spectral wavelength range (for colorless samples 190...400 nm is sufficient). Set step to 4 nm for normal use; set small step (and slit width) if high resolution of spectra with fine structure is wanted.
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