If the plate you are using is not found on the Recommended Mats and Vials you may configure a custom plate. Measure the exact dimensions of the plate as marked below and enter the values in the plate configuration table of the chromatographic data system.
Location | Description | Definition | Limits |
---|---|---|---|
|
Rows |
Number of rows on the plate |
up to 16 |
|
Columns |
Number of columns on the plate |
up to 24 |
|
Volume |
Volume (in µI) of a sample vessel |
|
A |
Row distance |
Distance (in mm) between the center of two rows |
|
B |
Column distance |
Distance (in mm) between the center of two columns |
|
C |
Plate length |
X size (in mm) at the bottom of the plate |
127.75+/- 0.25 mm (SBS Standard) |
D |
Plate width |
Y size (in mm) at the bottom of the plate |
85.50+/-0.25 mm (SBS Standard) |
E |
Plate height |
Size (in mm) from the bottom to the top of the plate |
up to 47 mm |
F |
Row offset |
Distance (in mm) from the back edge (bottom) to the center of the first hole (A1) |
|
G |
Column offset |
Distance (in mm) from the left edge (bottom) to the center of the first hole (A1) |
|
H |
Column shift |
Offset (in mm) to Y when the rows are not straight but staggered |
|
l |
Well diameter |
Diameter (in mm) of the well |
at least 4 mm |
J |
WeIl depth |
Distance (in mm) from the top of the plate to the bottom of the well |
up to 45 mm |
NOTE
The distances need to be measured with high precision. It is recommended to use calipers.
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