Step 3: Set Up Routine Methods

In routine analysis, sample matrices can have a significant influence on retention times. For reliable results, sample preparation must be thorough to avoid interferences or LC methods must be rugged enough. With difficult matrices, simultaneous multi-wavelength detection offers more reliability than timetable controlled wavelength switching. The FLD can, in addition, acquire fluorescence spectra while it records the detector signals for quantitative analysis. Therefore qualitative data are available for peak confirmation and purity checks in routine analysis.

In multi-wavelength mode, the FLD can acquire up to five different signals simultaneously. All of them can be used for quantitative analysis. Apart from complex matrices, this is advantageous when watching for impurities at additional wavelengths. It is also advantageous for reaching low limits of detection or increasing selectivity through optimum wavelength settings at any time. The number of data points acquired per signal is reduced and thus limits of detection may be higher, depending on the detector settings compared to the signal mode.