Method Development

Fluorescence detectors are used in liquid chromatography when superior limits of detection and selectivity are required. Thorough method development, including spectra acquisition, is fundamental to achieve good results. This chapter describes three different steps that can be taken with the Agilent 1290 Fluorescence Detector.

Subpages

Step 1: Check the LC System for Impurities
Step 2: Optimize Limits of Detection and Selectivity
Step 3: Set Up Routine Methods