Using the Agilent Lab Advisor
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Set up the HPLC system and the Agilent Lab Advisor.
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Flush the flow cell with clean bi-distilled water.
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Turn on the FLD lamp.
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Run the Wavelength Accuracy Test.
The FLD will change into the multi-excitation mode with emission wavelength at 397 nm and scan in the range of the expected maximum of 350 nm ± 20 nm.
As result, the maxima should be found at 350 nm ± 3 nm, see following figure.
The FLD will change into the multi-emission mode with excitation wavelength at 350 nm and scan in the range of the expected maximum of 397 nm ± 20 nm.
As result, the maxima should be found at 397 nm ± 3 nm, see following figure.
图: Excitation and Emission Spectrum (expected results) 注意
If the plots do not have a maximum around EM=397 nm and EX=350 nm (± 3 nm) the test fails. See Interpretation of the Results .
If the test fails observe the maxima of the EX or EM side under the Signals tab.
If the plots do not have a maximum around EX=397 nm and EX=350 nm (±3 nm) the test fails, see figure below. Refer to Interpretation of the Results .
base-id: 3586775947
id: 3586775947