Flush the Flow Cell
Quando
|
Ferramentas necessárias
Quant. | p/n | Descrição | |
|---|---|---|---|
1 | 
|
| Glass syringe |
1 |
|
| Adapter |
Peças necessárias
Quant. | p/n | Descrição | |
|---|---|---|---|
1 |
|
| Strong solvent |
1 |
|
| Tubings to waste |
ADVERTÊNCIA
Dangerous solvents
The strong solvents used in this procedure are toxic and flammable and proper precautions are necessary.
- Wear protective gloves and goggles.
- Don't expose yourself to the vapors.
NOTA
Aqueous solvents can build up algae and therefore should do not be left in the flow cell for long periods. Add a small percentage of organic solvents (e.g. acetonitrile or methanol ~ 5 %).
NOTA
The strong solvent should dissolve any potential contaminants in the flow cell. For example water for aqueous mobile phase buffers, chloroform or tetrahydrofuran for not water soluble contaminants.
NOTA
Do not exceed the flow cell pressure limit of 5 bar (0.5 MPa).
In case the cell is contaminated, follow the procedure below.
-
Use the purge mode and flush with the strong solvent.
-
Leave this solution in the cell for about one hour.
-
Flush with mobile phase.
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id: 3596214795