Replacing Capillaries on a Standard Flow Cell

Para módulos bio-inert, utilize somente peças bio-inert!

Quando

If the capillary is blocked

Ferramentas necessárias

Quant.	p/n	Descrição
1		Chave de 1/4 pol.
1	5043-0915	Ferramenta de montagem de encaixe
1		Wrench, 4 mm
1		Chave de fendas, Pozidriv Nº 1 PT3

Peças necessárias

Quant.		p/n	Descrição
1			For parts see Standard Flow Cell

Quant.

p/n

Descrição

For parts see

Standard Flow Cell

Pré-requisitos

Turn the lamp(s) off.
Open the doors of the module.
Remove the flow cell, see Remover e instalar uma Célula de fluxo.

NOTA

All descriptions in this procedure are based on the default orientation of the cell (as it is manufactured). The heat exchanger/capillary and the cell body can be fixed mirror symmetrically to have both capillaries routed to the bottom or to the top (depending on the routing of the capillaries to the column).

NOTA

The fittings at the flow cell body are special types for low dead volumes and not compatible with other fittings.

When retightening the fittings, make sure that they are carefully tightened (handtight plus 1/4 turn with a wrench). Otherwise damage of the flow cell body or blockage may result.

CUIDADO

Degradação da amostra e contaminação do instrumento

As peças metálicas na trajetória de fluxo podem interagir com as biomoléculas na amostra, o que provoca a degradação da amostra e contaminação.

Para aplicações bio, sempre use peças bio dedicadas, que possam ser identificadas pelo símbolo bio-inert ou outros marcadores descritos neste manual.
Não misture módulos ou peças bio e não bio em um sistema bio.

Remove the outlet capillary.
To replace the inlet capillary, use a 4 mm wrench to open the fitting.
Unscrew the cell body from the heat exchanger.
Remove the heat exchanger from the clamp unit.
Use a small flat screw driver to carefully lift off the I.D. tag. Shown is the default orientation. See Note at the beginning of this section.
Unscrew the fixing screw and unwrap the inlet capillary from the grove in the flow cell body.
Take the new inlet capillary and bend it 90° about 35 mm from its end.
Bend the capillary again by 90° as shown below.
Insert the capillary into the hole between fixing screw and the inlet fitting.
The capillary lays in the grove and should be tied around the body (in the grove) 5 times.
Insert the fixing screw, so that the capillary cannot leave the grove.
Carefully insert the I.D. tag into the new heat exchanger. Shown is the default orientation. See Note at the beginning of this section.
Fix the heat exchanger to the clamp unit.
Fix the flow cell body to the heat exchanger.
Fix the inlet capillary to the flow cell body handtight first. Then do a 1/4 turn with a 4 mm wrench.
After replacing the outlet capillary, fix it handtight first. Then do a 1/4 turn with a 4 mm wrench.
Check for a centered holder vs. hole. If required adjust with the holder screws.
Reconnect the capillaries, see Remover e instalar uma Célula de fluxo.
Perform a leak test.
Insert the flow cell.
Close the doors of the module.
Realize um Teste de Verificação de Comprimento de Onda ou um Teste de Óxido de Hólmio para verificar o posicionamento correto da lâmpada.

21-jun-2025