Replacing Capillaries on a Standard Flow Cell
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バイオイナートモジュールでは、バイオイナートの部品のみを使用してください。 |
実行するタイミング
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必要なツール
数量 | 部品番号 | 説明 | |
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1 | 
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| レンチ、1/4 インチ |
1 | | 5043-0915 | フィッティング取り付けツール |
1 |
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| Wrench, 4 mm |
1 |
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| ドライバ、Pozidriv #1 PT3 |
必要な部品
数量 | 部品番号 | 説明 | |
|---|---|---|---|
1 |
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| For parts see |
前提条件
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注記
All descriptions in this procedure are based on the default orientation of the cell (as it is manufactured). The heat exchanger/capillary and the cell body can be fixed mirror symmetrically to have both capillaries routed to the bottom or to the top (depending on the routing of the capillaries to the column).
注記
The fittings at the flow cell body are special types for low dead volumes and not compatible with other fittings.
When retightening the fittings, make sure that they are carefully tightened (handtight plus 1/4 turn with a wrench). Otherwise damage of the flow cell body or blockage may result.
注意
サンプルの劣化や機器の汚染に注意
流路内の金属部分がサンプルの生体分子と反応して、サンプルの劣化や汚染につながることがあります。
- バイオアプリケーションの場合は、必ずバイオ 部品を使用してください。バイオ部品は、本書のバイオイナートマークやその他のマークで識別できます。
- バイオシステムでは、バイオ用のモジュール/部品と、非バイオ用モジュール/部品を混在させないでください。
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Remove the outlet capillary.
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To replace the inlet capillary, use a 4 mm wrench to open the fitting.
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Unscrew the cell body from the heat exchanger.
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Remove the heat exchanger from the clamp unit.
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Use a small flat screw driver to carefully lift off the I.D. tag. Shown is the default orientation. See Note at the beginning of this section.
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Unscrew the fixing screw and unwrap the inlet capillary from the grove in the flow cell body.
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Take the new inlet capillary and bend it 90° about 35 mm from its end.
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Bend the capillary again by 90° as shown below.
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Insert the capillary into the hole between fixing screw and the inlet fitting.
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The capillary lays in the grove and should be tied around the body (in the grove) 5 times.
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Insert the fixing screw, so that the capillary cannot leave the grove.
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Carefully insert the I.D. tag into the new heat exchanger. Shown is the default orientation. See Note at the beginning of this section.
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Fix the heat exchanger to the clamp unit.
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Fix the flow cell body to the heat exchanger.
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Fix the inlet capillary to the flow cell body handtight first. Then do a 1/4 turn with a 4 mm wrench.
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After replacing the outlet capillary, fix it handtight first. Then do a 1/4 turn with a 4 mm wrench.
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Check for a centered holder vs. hole. If required adjust with the holder screws.
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Reconnect the capillaries, see フローセルの取り外し/取り付け.
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Perform a leak test.
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Insert the flow cell.
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Close the doors of the module.
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波長ベリフィケーションテストまたはホルミウムテストを実行し、ランプが正しい位置に取り付けられていることを確認します。
base-id: 3358984715
id: 3358984715