Method Parameter Settings (Hybrid Multisampler)
These settings are available via the Method Ribbon tab or via right click the Active area of the corresponding Instrument Dashboard.
Injection Mode | Injection Mode It is possible to select between Classical flow-through and Feed Injection mode. |
Injection volume | Injection The settable Injection volume is depending on what kind of configuration is installed. Default configuration 0.1 – 100 µL. |
Stoptime and Posttime | Stoptime/Posttime An Online Sample Manager Stoptime can be set. For equilibration of the Online Sample Manager a Posttime can be set. |
NOTE
Usually default draw offset = 0 equates to 2 mm above the wellplate bottom.
NOTE
For help and support, highlight the desired cell and press the F1 key. A help screen will open with additional information and documentation about the topic.
Specific Feed Injection Parameter Details | Specific Feed Injection Parameter Details Feed Speed The Feed Speed influences the sample dilution degree during injection into the mobile phase flow path, affecting peaks shape. Selectable options:
Flush Out The Flush Out influences the carryover and sample recovery from the injection flow path. Selectable options:
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NOTE
For further details about Feed Injection parameter optimization, see chapter Optimizing Performance.
Specific flow-through injection Parameter Details | Specific Flow-through Injection Parameter Details Injection Valve to Bypass for Delay Volume Reduction Automatic Delay Volume Reduction (ADVR) helps to optimize the injection cycle to reduce the system delay volume related to the injection flow path. As a result, also to decrease retention time for sample components. Sample Flush-Out Factor The Sample Flush-Out Factor defines the moment, in which the sampler switches from main pass to bypass for delay volume reduction after injection. |
NOTE
For further details about Flow-through Injection parameter optimization, see chapter Optimizing Performance.
Injection Path Cleaning Wash Options | Injection Path Cleaning It is possible to choose between different Wash Options:
The Wash Options provide different combinations of wash steps, which can be performed in addition to a basic Reconditioning step. The Reconditioning step flushes the flow path with Feed/Flush-out Solvent (S2) to restore it to the initial default condition. Besides the default settings, you can change which solvent is used. The step is fixed and cannot be adjusted in terms of volume and duration. The Reconditioning happens:
The Inner wash enables an additional wash step for the flow path. You can choose the solvent type, solvent volume, and duration of the step. The Outer wash is a wash step for the outer Needle surface in the Wash port. You can choose the solvent type, solvent volume, and duration of the step. The Seat wash is a wash step for the surface of the Needle Seat. You can choose the solvent type, solvent volume, and duration of the step. |
NOTE
It takes approximately 30 s to fully exchange one solvent for another in the flush port. To flush and exchange the solvent in the needle seat, it takes 18 s.
Also it is highly recommended to use Auto-Clean function to flush the module regularly with all installed solvents.
Flow-through injection, standard wash option
Feed Injection, standard wash option
| Depending on the selected Wash Option and Injection Mode, the sequence of the wash steps is different. If Off is selected for Inner Wash Mode and Outer Wash Mode, only the Reconditioning step cleans the flow path. Standard is selected for Inner Wash Mode and Outer Wash Mode:
Extended is selected for Inner Wash Mode and Outer Wash Mode:
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