The Hybrid Multisampler employs two injection mechanisms, Feed Injection and classical flow-through injection.
The Hybrid Multisampler transport mechanism uses a Cartesian robot. The X-Y drive coupled with the Z-drive optimize the grabbing and positioning for the sample trays and the needle handling inside the Hybrid Multisampler. The sample coupler moves the sample container from the sample hotel, which stores all the samples, and places it on the central workspace. Then, the needle coupler of the Z-drive takes over and performs the analytical procedures inside the Hybrid Multisampler. Due to the uncoupled needle design, the robot can do other liquid handling jobs during the analysis.
The Hybrid Multisampler employs an active vial or plate pusher mechanism to hold down the vial or plate while the needle is drawn back from the sample vessel. If a septum is used, this mechanism is a must. The active vial or plate pusher employs a sensor to detect the presence of a plate and to ensure accurate movement regardless of the plate used. Fast BLCD motors drive all axes of the transport mechanism. Optical encoders ensure the correct operation of the movement.
The Hybrid Multisampler has a 100 µL metering device, which enables a maximum injection volume of 100 µL. For minimum internal carryover, the mobile phase flushes the entire injection flow path.
In addition, the external needle wash reduces the carryover. After drawing the sample into the loop, the needle coupler moves the needle into the wash port. A micro piezo pump combined with a solvent selection valve washes the outside of the needle. This step reduces the already low carryover for sensitive analysis. You can select between two different solvents for the needle wash.
A high-speed hybrid stepper motor drives the six-port injection valve unit. During the sampling sequence, the valve unit bypasses the Hybrid Multisampler, and connects flow from the pump directly to the column. For analysis, it is crucial that the sample is injected to the column completely and that the flow path is clean. Depending on the method, different mechanisms ensure this necessity:
In classical flow-through injection mode, the valve unit directs the flow through the entire sampler flow path.
In Feed Injection mode, the flush head rinses the metering device, loop, needle, and needle seat.
The temperature control of the vial or plate in the Hybrid Multisampler is achieved using an Agilent InfinityLab Sample Thermostat module. The chiller unit is a micro compressor-based refrigerator. A fan draws air from the central workstation above the sample container of the Hybrid Multisampler. It is then blown through the fins of the thermostatting module, in which it is adjusted according to the temperature settings. The cooled air enters the Sample Hotel through a recess underneath a specially designed base plate. The air is then distributed evenly through the Sample Hotel ensuring effective temperature control, regardless of the number of samples in the drawer. In cooling mode, condensation is generated on the cooling side of the Sample Thermostat. The condensate is safely guided into a waste bottle for condensed water, which is located underneath the working bench.
base-id: 6301611531
id: 9007205556352523