Always place the solvent cabinet with the solvent bottles on top of the quaternary pump (or at a higher level).
When using salt solutions and organic solvents in the quaternary pump it is recommended to connect the salt solution to one of the bottom gradient valve ports and the organic solvent to one of the upper gradient valve ports. It is best to have the organic channel directly above the salt solution channel. Regular flushing of all MCGV/4CGV channels with water is recommended to remove all possible salt deposits in the valve ports.
Before operating the quaternary pump, flush the pump and vacuum degasser. This is especially recommended if it has been turned off for some time (for example, overnight) and volatile solvent mixtures are used in the channels.
Prevent blocking of solvent inlet filters. Never use the pump without solvent inlet filter. Prevent the growth of algae, see Prevent Blocking of Solvent Filters).
Regularly check the purge valve frit and column frit. A blocked purge valve frit can be identified by a black or yellow surface, deposits or by a pressure greater than 10 bar, when pumping distilled water at a rate of 5 mL/min with an open purge valve.
When using the quaternary pump at low flow rates (for example, 0.2 mL/min) check all 1/16-inch fittings for any signs of leaks.
Whenever exchanging the pump seals the purge valve frit should be exchanged, too.
When using buffers or other salt solutions, flush the system with water before switching it off. The seal wash option should be used when salt concentrations of 0.1 M or higher will be used for long time periods.
Check the pump pistons for scratches when changing the piston seals. Scratched pistons will cause micro leaks and will decrease the lifetime of the seal.
Pressurize the system according to the wear in procedure after changing the piston seals (see Maintenance of a Pump Head Without Seal Wash Option).
Consider recommendations given in the solvent information section, see Solvent Information.
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