The pump method setup parameters are in eight sections:
Flow
Solvent(s)
Stoptime
Posttime
Pressure Limits
Timetable
Advanced
Parameter | Limits | Description |
|---|---|---|
Flow | ≤ 200 bar: 0.0 – 10.00 mL/min in steps of 0.001. > 200 bar: 0.0 – 5.00 mL/min in steps of 0.001. | The flow is the rate of movement of eluent along the column. It is important that the flow rate is kept constant to ensure precise retention time, and peak measurements. Variations in flow rate can occur as a result of the following: partial failure of the pumping system, air in the pumping system, a change in the mobile phase viscosity, or a temperature change. |
Solvent(s) |
| Isocratic Pump: The text box allows you to type a brief description of the solvent. Quaternary Pump: You can set the percentages of solvents B, C, and D to any value from 0 through 100 %. Solvent A always delivers the remaining volume: 100 - (%B + %C + %D). The check boxes allow you to turn on the solvent channels (checked) or off (cleared). The text boxes allow you to type a brief description of each of the solvents. |
Stoptime | 0.01 – 99999 minor As Injector/No Limit (an infinite run time). | The stoptime sets a time limit for your analysis. After the stoptime, all gradients are stopped and the pump parameters return to their initial values. The pump can be used as a stoptime master for the complete analytical system. The pump also stops the detectors if they have a No Limit stoptime setting. If no limit is given, a method will have to be stopped manually. |
Posttime | 0.01 – 99999 minor Off ( 0.0 min). | Your instrument remains in a not ready state during the posttime to delay the start of the next analysis. You can use the Posttime to allow your column to equilibrate after changes in solvent composition (for example after gradient elution). |
Pressure Limits | Max: 600 bar (8700 psi) for flow rates up to 5 mL/min. 200 bar (2900 psi) for flow rates > 5 mL/min. Min: any value between 0 and the upper pressure limit setting. | Sets the maximum and minimum pressure limits for the pump.
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Timetable |
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Advanced |
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The pump advanced method setup parameters are in four sections:
Minimum Stroke
Compressibility
Maximum Flow Gradient
Primary Channel (Quaternary Pump only)
Parameter | Limits | Description |
|---|---|---|
Minimum Stroke | 20 – 100 µL | The Stroke Volume is used for optimizing between performance of the module and seal life time. For performance a low stroke volume is beneficial, as it divides disturbances into smaller packages, but a larger volume is extending the life time of the pump seals. If Automatic is activated, the pump tries to achieve an optimized stroke volume for best performance and lowest ripple. |
Compressibility | 40·10-6 – 150·10-6 . Default compressibility value: 100·10-6 | The compressibility of the mobile phase has an effect on the performance of the pump. For best flow accuracy and mixing performance, you can set the parameter according to the mobile phase being used. |
Maximum Flow Gradient | 0.1 – 100 mL/min/min Default value: 100 mL/min/min | You can set a limit on the rate of change of the solvent flow to protect your analytical column. |
Primary Channel (Quaternary pump only) |
| Using Automatic is recommended. The primary channel can be specified as A to D for optimizing highly specific methods. It is split up to deliver the first and last solvent package created by the MCGV/4CGV in order to optimize composition precision. The primary channel does not change during a gradient, as long as the channel is used. Using Automatic chooses the channel with the highest percentage at start conditions before a gradient. |
Use the Timetable to program changes in the pump parameters during the analysis by entering a time in the Time field and appropriate values in the following fields of the timetable. Changes in flow rate occur linearly from either time zero or the time of the last defined change; other parameters change instantaneously at the time defined in the timetable.
Show Advanced Timetable toggles the timetable display between standard mode and advanced mode.
The following parameters can be changed:
Change Contacts
Change Flow
Change Max. Pressure Limit
Change Solvent Composition - You can only use solvents, which have been enabled in the solvents section.
Function centric view - This checkbox allows you displaying parameter changes instead of a time table.
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