Method Parameters

The Method dialog box enables the user to change the method parameters relevant for the ICC:

• Temperature:

  • Not Controlled: The temperature of the column oven stays uncontrolled.

  • At specified temperature (°C): The temperature of the column oven is regulated to stay at the setpoint temperature specified.

    NOTE

    Valid inputs are 10  to 80 °C in increments of 0.1 °C.

  • Unchanged: The temperature setting stays unchanged, meaning that the setting currently stored in the system is used by the method.

• Valve Position/Column: This section provides information about the location of the column that is being used for the given method.

  • Enforce column for run: Marking this checkbox restricts the execution of the method to a specific column type, identified by its product number.

• Stoptime:

  • As Pump/Injector: The ICC uses the same stoptime setting as specified for the pump or the sampler.

  • After specified time (min): The stop time defines a time limit for the analysis, after which all method parameters are reset to initial values.

• Posttime:

  • Off: No post-run period is applied.

  • For specified time (min): After the analysis, the system stays in the post-run state for the specified time. Using a post-time period can be beneficial when the column needs to be re-equilibrated before starting the next analysis (for example, gradient elutions).

    NOTE

    For Stoptime and Posttime, valid inputs are 0.01  to 99999  minutes in increments of 0.1 min.

• Enable Analysis:

  • With any temperature: The analysis starts regardless of the actual column oven temperature.

  • When temperature is within: The analysis starts only when the actual column oven temperature is within the specified range of the setpoint temperature.

• Timetable: This feature enables the user to use programmed changes to control the oven temperature during the course of the analysis.