NOTE
New instruments are shipped with a flow cell installed and have a factory wavelength calibration.
If a flow cell is used other than the installed/shipped flow cell:
verify that the installed flow cell is fixed very tight,
perform a wavelength calibration using Glycogen (part of FLD Calibration Kit (G7121-68001)).
The wavelength calibration is based on a Glycogen solution, which acts as a strong elastic light scatterer (refer to ASTM Test Method E388-72-1993 “Spectral Bandwidth and Wavelength Accuracy of Fluorescence Spectrometers”). The Glycogen solution is introduced into the flow cell and then the built-in wavelength calibration functionality is used.
The algorithm is based on evaluating different grating orders and calculating the wavelength scales of both, excitation and emission monochromator, by applying the fundamental grating equation.
NOTE
A complete wavelength calibration is not always required. In most cases a quick wavelength accuracy verification is sufficient enough, see Reasons for doing a Verification or Calibration.
| Verification | WL calibration |
|---|---|---|
interest | X |
|
GLP compliance | X |
|
cell change |
| X |
lamp change |
| X |
monochromator change |
| X |
main board change |
| X |
optical unit change |
| X |
|
communication board change |
| X |
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