Cell Test
The Cell Test compares the intensity of the deuterium lamp measured by the sample and reference diodes (unfiltered and not logarithmized) when the grating is in the zero-order position. The resulting intensity ratio (sample:reference) is a measure of the amount of light absorbed by the flow cell.
The test can be used to check for dirty or contaminated flow cell windows. When the test is started, the gain is set to -1. To eliminate effects due to absorbing solvents, the test should be done with water in the flow cell.
Limits: No real limit. The reason is that it depends on the position/alignment of the reference side (beam splitter – reference slit – reference diode). Therefore the reference side value can be higher/smaller than the sample side value.
With a clean cell the counts for sample and reference (photocurrent) are in the same range. If the sample side shows much lower values than the reference side the flow cell might have a problem.
Prerequisites
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Run the Cell-Test with Agilent Lab Advisor (for further information see Online-Help of user interface).
Figure: Test – Results
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