When the solvents have been exchanged or the pumping system has been turned off for a certain time (for example, overnight) oxygen will re-diffuse into the solvent channel between the solvent reservoir, vacuum degasser (when available in the system) and the pump. Solvents containing volatile ingredients will slightly lose these. Therefore priming of the pumping system is required before starting an application.
Activity | Solvent | Comments |
|---|---|---|
After an installation | Isopropanol | Best solvent to flush air out of the system |
When switching between reverse phase and normal phase (both times) | Isopropanol | Best solvent to flush air out of the system |
After an installation | Ethanol or Methanol | Alternative to Isopropanol (second choice) if no Isopropanol is available |
To clean the system when using buffers | Bidistilled water | Best solvent to re-dissolve buffer crystals |
After a solvent change | Bidistilled water | Best solvent to re-dissolve buffer crystals |
After the installation of normal phase seals (P/N 0905-1420) | Hexane + 5% Isopropanol | Good wetting properties |
NOTE
The pump should never be used for priming empty tubings (never let the pump run dry). Use a syringe to draw enough solvent for completely filling the tubings to the pump inlet before continuing to prime with the pump.
Open the purge valve of your pump (by turning it counterclockwise) and set flow rate to 3 – 5 mL/min.
Flush all tubes with at least 30 mL of solvent.
Set flow to required value of your application and close the purge valve.
NOTE
Pump for approximately 10 minutes before starting your application.
NOTE
Do NOT exceed the flow rate of the 1290 Infinity III RID (Micro) whilst purging pump. Bypass detector when purging >1 mL/min.
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