The Hybrid Multisampler processor continuously monitors the movements of the sampler components during the sampling sequence. The processor defines specific time windows and mechanical ranges for each movement. If a specific step of the sampling sequence is not completed successfully, an error message is generated. During the sampling sequence, the injection valve bypasses the Hybrid Multisampler. The sample coupler moves the required sample container automatically from the sample hotel to the central workspace. Then, the needle assembly moves via robot to the desired position and lowers into the sample liquid. The metering device draws the desired sample volume by moving its plunger back a certain distance. The needle assembly raises out of the sample and moves into the wash port for external needle wash. Afterwards, the needle assembly moves to the needle park position in the seat and the sample injection starts.
Sampling sequence for Feed Injection:
The robot loads the required sample container on the central workspace.
The injection valve switches to the bypass position.
The plunger of the metering device moves to the initialization position, pushing out clean eluent through the needle.
The robot couples into the needle assembly from the needle park station.
The robot unlocks the needle assembly and moves up.
The coupled needle assembly/robot moves to the desired sample vial (or well plate) position on the central workstation.
The needle lowers into the sample vial (or well plate).
The metering device draws the preset sample volume.
The needle lifts out of the sample vial (or well plate).
Optional external needle wash in the wash port.
The coupled needle assembly/robot is then moved to the park station onto the seat to close the sample loop.
The needle assembly is locked into the park station and moves down.
The injection valve switches to Feed position.
The metering device pushes the sample into the mobile phase stream.
The injection valve switches to flush position, in which the mobile phase stream bypasses the sampler and the flush head and metering device are connected to the needle assembly.
The flush head draws the preset volume of flush solvent and pushes it through the whole injection path towards the waste to clean the injection path from sample remaining.
If the sampling sequence is done, the robot moves the sample container back into the sample hotel.
Sampling sequence for classical flow-through injection:
The robot loads the required sample container on the central workspace.
The injection valve switches to the bypass position.
The plunger of the metering device moves to the initialization position, pushing out clean eluent through the needle.
The robot couples into the needle assembly from the needle park station.
The robot unlocks the needle assembly and moves up.
The coupled needle assembly/robot moves to the desired sample vial (or well plate) position on the central workstation.
The needle lowers into the sample vial (or well plate).
The metering device draws the preset sample volume.
The needle lifts out of the sample vial (or well plate).
Optional external needle wash in the wash port.
The coupled needle assembly/robot is then moved to the park station onto the seat to close the sample loop.
The needle assembly is locked into the park station and moves down.
The injection cycle is completed when the injection valve switches to the mainpass (main path) position. The sample is transported onto the column with the solvent stream.
If the sampling sequence is done, the robot moves the sample container back into the sample hotel.
base-id: 6301223691
id: 6301223691