Supercritical fluid chromatography (SFC) was first introduced by Klesper et al. in 1962 (Klesper, E.; Corwin, A. H.; Turner, D. A. J. Org. Chem. 1962, 27,700.) for the separation and analysis of a porphyrin mixture using open tubular SFC. The first commercial instruments using packed columns were available from Hewlett-Packard (HP) in 1982. Since then, several vendors have developed and commercialized packed column SFC instrumentation for analytical as well as for preparative separation. SFC is widely accepted for the separation of chiral compounds and increased user interest has been observed for a wide spectrum of small to medium sized molecules due to the analysis speed achieved and the low solvent consumption.
The latest introduction of analytical SFC instrumentation, the Agilent 1260 Infinity III SFC Control Module can be coupled to a Binary LC system optimized for SFC.
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