In contrast to reversed phase separation, there is no universal stationary phase available for SFC separations. Most typically used stationary phases are ethyl pyridine, diol, cyano, amino, Silica and SCX columns. This usually leads to additional effort to screen different columns in order to achieve optimum separation. On the other hand, it provides a valuable tool for achieving different selectivities for a given analyte mixture. A review of column developments for SFC was recently published by T.Berger, B.Berger & R.E.Majors in LCGC North America, May 1, 2010.
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