SFC is widely accepted for the analysis and separation of chiral compounds. In addition, it gains increasing acceptance as a complementary liquid-based separation technique to HPLC for high-throughput and high-resolution analysis of complex mixtures. This is due to the thermodynamic properties of supercritical fluids, which can be exploited for high throughput and high efficiency. In addition, the mild thermal conditions also allow for the analysis of thermally labile compounds. Typically, analysis times and column re-equilibration are decreased by a factor of 3–5 compared to standard HPLC. With the increasing costs of organic solvents and the environmental awareness to minimize toxic waste, production SFC is increasingly accepted as the “green alternative” to normal phase or reversed phase chromatography, gaining popularity in method development and UV- and MS-based separation and purification. A variety of parameters, such as stationary phase selection, mobile phase composition, modifier type and concentration, column temperature and system pressure, can be easily manipulated to fulfill separation requirements by influencing, optimizing and exploiting selectivity in SFC.
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