This procedure exemplarily shows how to prepare a run. Parameters as shown in the screenshots may vary, depending on the system installed.
WARNING
Toxic, flammable and hazardous solvents, samples and reagents
The handling of solvents, samples and reagents can hold health and safety risks.
Switch on the detector.
Fill the solvent bottles with adequate solvents for your application.
Place solvent tubings with bottle head assemblies into the solvent bottles.
Place solvent bottles into the solvent cabinet.
Solvent bottle filling dialog (in the software).
Solvent bottle filling dialog (in the software).
Purge the pump.
NOTE
For details on priming and purging, refer to the technical note Best Practices for Using an Agilent LC System Technical Note (InfinityLab-BestPractice-en-SD-29000194.pdf, SD-29000194).
Change solvent type if necessary.
Choose the tray format of the sampler.
Add a new column.
Enter the column information.
Select the column in the Method settings of the column compartment.
Set the detector parameters according to the needs of your method.
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