Flush the column with the appropriate solvents and store it according to column manual instructions (be sure that the flushing solvent is compatible with the solvent present in the system to avoid precipitation).
Chromatographic columns used for biomolecule separations like size exclusion chromatography or ion-exchange chromatography should be stored in the presence of 0.02 % sodium azide. For more Bio LC Column user guides, see https://www.agilent.com/en/support/liquid-chromatography/kb005960.
Install a union or a restriction capillary and flush the system extensively with water, especially after using buffers. For details, see Best Practices for Using an Agilent LC System Technical Note (InfinityLab-BestPractice-en-SD-29000194.pdf, SD-29000194) or Agilent InfinityLab LC Series High-Speed Pumps User Manual (G7120-G7132-HighSpeedPump-UseMa-en-SD-29000230.pdf, SD-29000230).
Flush and store the system in 50 % methanol or 50 % isopropanol in water, without additives.
Remove all samples from the autosampler and store them according to good laboratory practices.
Power off all modules.
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