If you only have small quantities of sample, this is the method of choice. In the partialfilling method the volume of sample injected is set by the syringe. In this method, no more than half a loop volume of sample should be loaded into the loop. For example, load no more than 1 mL into a 2 mL loop. With larger than half the loop volume, some of the sample is lost out Vent Line 6. This is because sample flows down the center of the loop at twice the average velocity due to the laminar flow effect.
In INJECT, use the Needle Port Cleaner to flush out the needle port with about 1 mL of mobile phase to flush out contamination from the earlier injection. This liquid will exit out Vent Line 5.
Insert the syringe into the needle port. You should feel slight resistance as the needle passes through the needle seal before it stops against the stator face.
Load the sample slowly onto the loop.
Leave the syringe in and turn the handle to INJECT.
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