In complete-filling, the volume of sample injected is set by the volume of the loop (this includes the valve passages). This method produces the highest precision.
3 x when loop <= 100 µL
2 x when loop = 100 – 500 µL
1.5 x when loop > 500 µL
At least 2 to 3 sample-loop volumes (more if better precision is required) of sample should be injected through the needle port to provide good precision.
The sample fills the loop, and excess sample is expelled through the vent tube connected to port 6.
An excess of sample is needed because mobile phase near the wall of the loop is displaced slowly due to the laminar flow effect.
Turn the handle to the LOAD position
Insert the syringe into the needle port. You should feel slight resistance as the needle passes through the needle seal before it stops against the stator face.
Load the sample slowly onto the loop. Repeat this step for higher precision.
Leave the syringe in and turn the handle to INJECT.
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