It is recommended to use a high-performance pumping system with no flow pulses to minimize nebulization problems. Inconsistent solvent flow will result in poor reproducibility.
A backpressure regulator maybe necessary on certain pumps to minimize pulsation. This can also be achieved by the column itself or a coil of 0.127 mm (0.005 in) ID tubing placed between the pump and the injector/injection valve.
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id: 4343447179