Noisy baseline

Error ID:

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Probable cause		Suggested actions
1	NOTE: Due to its miscibility with water and most organic solvents, isopropanol is often the choice when removing air bubbles from an HPLC system.	Degas mobile phases. Purge the pump and system with a viscous mobile phase to remove any air bubbles.
2	Improper method settings	Check the detector method settings , such as wavelength and data rate, and optimize them if needed.
3	Aging detector lamp	Check if the on-time hours of the detector lamp have exceeded the expected lifetime. Run detector lamp intensity test to check the condition of the lamp. Replace the lamp if necessary.
4	NOTE: The eluents should be miscible and should not form salt precipitates over the range of the entire gradient. The eluents should also be properly degassed. NOTE: Due to its miscibility with water and most organic solvents, isopropanol is often the choice when removing air bubbles from an HPLC system.	Ensure that the method settings are properly set and the gradient system is delivering the correct gradient composition. Check if the mobile phase reservoirs are empty or if the filter frits are blocked. Check for air bubbles in the system. If necessary, purge the pump and system with a viscous mobile phase to remove any air bubbles. Check the system for any leaks. Check for leaking pump seals. If necessary, replace pump seals. Check for sticking check valves. If necessary, sonicate or replace check valves.
5	Detector cell contamination	Inspect the flow cell for salt crystals, air bubbles, or contamination. If necessary, clean/flush the flow cell or replace it.
6	NOTE: Consider changing the mobile phase mixer in the system flow path for generating more homogeneous mobile phases and more consistent gradient formations.	Ensure mobile phase additives, such as TFA, are homogeneously mixed before reaching the detector flow cell.
7	Mobile phase miscibility	Ensure that the mobile phases are completely miscible.
8	Electrical interference	Ensure that the power supply to the LC system is stable. Check for local interfering sources. If possible, use an independent electrical current for the LC System.

21-Jun-2025