The High-Speed Pump method setup parameters are in eight sections:
Flow
Solvents A and B
Stoptime
Posttime
Pressure Limits
Timetable
Advanced
External Contacts
Parameter | Limits | Description |
|---|---|---|
Flow | 0.00 – 5.00 mL/min in steps of 0.001. Recommended flow range: 0.05 – 5.00 mL/min. | The flow is the rate of movement of eluent along the column. It is important that the flow rate is kept constant to ensure precise retention time and peak measurements. Variations in flow rate can occur as a result of the partial failure of the pumping system, air in the pumping system, a change in the mobile phase viscosity or a temperature change. |
Solvents A and B |
| For each channel, you can select which of the two solvents to deliver. You can set the percentage of solvent B to any value from 0 through 100 %. Solvent A always delivers the remaining volume: 100 - %B. The solvent B check boxes allow you to turn the solvent B channels on (checked) or off (cleared). When the Use solvent types check box in the Compressibility section is checked (see Advanced Settings), you click the down arrow and select either a Generic solvent or a calibrated Solvent. |
Stoptime | 0.01 – 99999 min or As Injector/No Limit (an infinite run time). | The stoptime sets a time limit for your analysis. After the stoptime, all gradients are stopped and the pump parameters return to their initial values. The pump can be used as a stoptime master for the complete analytical system. The pump also stops the detectors if they have a No Limit stoptime setting. If no limit is given, a method will have to be stopped manually. |
Posttime | 0.01 – 99999 min or Off (0.0 min). | Your instrument remains in a not ready state during the posttime to delay the start of the next analysis. You can use the Posttime to allow your column to equilibrate after changes in solvent composition (for example, after gradient elution). |
Pressure Limits | Max: 1300 bar (18850 psi) for flow rates up to 2 mL/min. For flow rates between 2 mL/min and 5 mL/min, the maximum pressure ramps down to 800 bar (11600 psi). Min: any value between 0 and the upper pressure limit setting. | Sets the maximum and minimum pressure limits for the pump.
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Timetable |
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Advanced |
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External Contacts |
| The External Contacts section enables you to set up the switching of the external contacts.
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The High-Speed Pump advanced method setup parameters are in three sections:
Minimum Stroke
Compressibility
Maximum Flow Gradient
Parameter | Limits | Description |
|---|---|---|
Minimum Stroke | 20 – 100 µL | The stroke volume is used for optimizing between performance of the module and seal life time. For performance, a low stroke volume is beneficial, as it divides disturbances into smaller packages, but a larger volume extends the lifetime of the pump seals. If Automatic is selected, the pump tries to achieve an optimized stroke volume for the Jet Weaver geometry. Synchronized: Select this option to synchronize the strokes for both channels; the values for Channel B are set to the same as those for Channel A. This is done to avoid floating disturbances affecting instrument performance. |
Compressibility |
| The compressibility of the mobile phase affects the performance of the pump. For best flow accuracy and mixing performance, you can set the parameter according to the mobile phase being used. Use solvent types:
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Maximum Flow Gradient | 1.000 – 1000.000 mL/min/min in steps of 0.001 mL/min/min Default value: 100.000 mL/min/min | You can set a limit on the rate of change of the solvent flow to protect your analytical column. You can set individual values for Flow ramp up and Flow ramp down. |
Use the Timetable to program changes in the pump parameters during the analysis by entering a time in the Time field and appropriate values in the following fields of the timetable. Changes in flow rate occur linearly from either time zero or the time of the last defined change; other parameters change instantaneously at the time defined in the timetable.
Show that Advanced Timetable toggles the timetable display between standard mode and advanced mode.
The following parameters can be changed:
Change Contacts
Change Flow
Change Max. Pressure Limit
Change Solvent Composition - You can only use solvents, which have been enabled in the solvents section.
Function centric view - This check box allows you to display parameter changes instead of a time table.
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id: 4202927499