Recommendations for Setting up Methods Using Salt-Containing Eluents

For LC Drivers 3.4 or newer, please use Ammonium Sulfate in Water V.04 and Sodium Chloride in Water V.04 and manually input the molarity (0.5 M - 3.0 M or 0.5 M - 2.0 M respectively) as shown in the example below.

With the 1290 Infinity III Bio LC System, Agilent offers a dedicated instrument for running applications that are typical for the (Bio)pharma market, such as IEX (Ion Exchange Chromatography) or HIC (Hydrophobic Interaction Chromatography), and applications in Academia, Government and Applied Markets, using concentrated salt solutions as eluents.

For LC Drivers 3.3 or older, please choose the solvent definition most suitable for the application according to the chart below.

NOTE

Ammonium Sulphate V.03 and Sodium Chloride V.03 only properly characterize the G7132A High-Speed Pump. Do not use these with any quaternary/flexible pump configurations.

Main salt component	Total salt content (c, mol/L)	Select solvent definition
NaCl	0.25 < c ≤ 0.75	Sodium Chloride 0.5 M V.03
	0.75 < c ≤ 1.25	Sodium Chloride 1.0 M V.03
	1.25 < c ≤ 1.75	Sodium Chloride 1.5 M V.03
	c > 1.75	Sodium Chloride 2.0 M V.03
(NH₄)₂SO₄	0.25 < c ≤ 0.75	Ammonium Sulphate 0.5 M V.03
	0.75 < c ≤ 1.25	Ammonium Sulphate 1.0 M V.03
	1.25 < c ≤ 1.75	Ammonium Sulphate 1.5 M V.03
	1.75 < c ≤ 2.5	Ammonium Sulphate 2.0 M V.03
	c >2.5	Ammonium Sulphate 3.0 M V.03

21-Jun-2025