Highly concentrated buffer solutions will reduce the lifetime of the seals and pistons in your pump. The seal wash function allows to maintain the seal lifetime by flushing the back side of the seal with a wash solvent.
The seal wash function is strongly recommended when buffer concentrations of 0.1 M or higher will be used for long time periods in the pump.
The seal wash function comprises a support ring, secondary seal, gasket and seal holder for both piston sides. Place a wash bottle filled with 90 % water / 10 % isopropanol above the pump in the solvent cabinet and guide the SW waste into the waste bottle underneath the LC stack. The peristaltic pump moves a flow through the pump head removing all possible buffer crystals from the back of the pump seal. This mixture prevents growth of algae or bacteria in the wash bottle and reduces the surface tension of the water.
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